A quantitative assay for reductive metabolism of a pesticide in fish using electrochemistry coupled with liquid chromatography tandem mass spectrometry.
Identifieur interne : 000153 ( Main/Exploration ); précédent : 000152; suivant : 000154A quantitative assay for reductive metabolism of a pesticide in fish using electrochemistry coupled with liquid chromatography tandem mass spectrometry.
Auteurs : Ugo Bussy [États-Unis] ; Yu-Wen Chung-Davidson [États-Unis] ; Ke Li [États-Unis] ; Weiming Li [États-Unis]Source :
- Environmental science & technology [ 1520-5851 ] ; 2015.
English descriptors
- KwdEn :
- Animals, Chromatography, Liquid (methods), Electrochemical Techniques (methods), Female, Fishes (metabolism), Male, Nitrophenols (analysis), Nitrophenols (pharmacokinetics), Oncorhynchus mykiss (metabolism), Pesticides (analysis), Pesticides (pharmacokinetics), Petromyzon (metabolism), Species Specificity, Tandem Mass Spectrometry (methods).
- MESH :
- chemical , analysis : Nitrophenols, Pesticides.
- metabolism : Fishes, Oncorhynchus mykiss, Petromyzon.
- methods : Chromatography, Liquid, Electrochemical Techniques, Tandem Mass Spectrometry.
- chemical , pharmacokinetics : Nitrophenols, Pesticides.
- Animals, Female, Male, Species Specificity.
Abstract
This is the first study to use electrochemistry to generate a nitro reduction metabolite as a standard for a liquid chromatography-mass spectrometry-based quantitative assay. This approach is further used to quantify 3-trifluoromethyl-4-nitrophenol (TFM) reductive metabolism. TFM is a widely used pesticide for the population control of sea lamprey (Petromyzon marinus), an invasive species of the Laurentian Great Lakes. Three animal models, sea lamprey, lake sturgeon (Acipenser fulvescens), and rainbow trout (Oncorhynchus mykiss), were selected to evaluate TFM reductive metabolism because they have been known to show differential susceptibilities to TFM toxicity. Amino-TFM (aTFM; 3-trifluoromethyl-4-aminophenol) was the only reductive metabolite identified through liquid chromatography-high-resolution mass spectrometry screening of liver extracts incubated with TFM and was targeted for electrochemical synthesis. After synthesis and purification, aTFM was used to develop a quantitative assay of the reductive metabolism of TFM through liquid chromatography and tandem mass spectrometry. The concentrations of aTFM were measured from TFM-treated cellular fractions, including cytosolic, nuclear, membrane, and mitochondrial protein extracts. Sea lamprey extracts produced the highest concentrations (500 ng/mL) of aTFM. In addition, sea lamprey and sturgeon cytosolic extracts showed concentrations of aTFM substantially higher than those of rainbow trout. However, other fractions of lake sturgeon extracts tend to show aTFM concentrations similar to those of rainbow trout but not with sea lamprey. These data suggest that the level of reductive metabolism of TFM may be associated with the sensitivities of the animals to this particular pesticide.
DOI: 10.1021/es5057769
PubMed: 25730707
Affiliations:
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Le document en format XML
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of a pesticide in fish using electrochemistry coupled with liquid chromatography tandem mass spectrometry.</title><author><name sortKey="Bussy, Ugo" sort="Bussy, Ugo" uniqKey="Bussy U" first="Ugo" last="Bussy">Ugo Bussy</name><affiliation wicri:level="4"><nlm:affiliation>Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824</wicri:regionArea><orgName type="university">Université d'État du Michigan</orgName><placeName><settlement type="city">East Lansing</settlement><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Chung Davidson, Yu Wen" sort="Chung Davidson, Yu Wen" uniqKey="Chung Davidson Y" first="Yu-Wen" last="Chung-Davidson">Yu-Wen Chung-Davidson</name><affiliation wicri:level="4"><nlm:affiliation>Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824</wicri:regionArea><orgName type="university">Université d'État du Michigan</orgName><placeName><settlement type="city">East Lansing</settlement><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Li, Ke" sort="Li, Ke" uniqKey="Li K" first="Ke" last="Li">Ke Li</name><affiliation wicri:level="4"><nlm:affiliation>Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824</wicri:regionArea><orgName type="university">Université d'État du 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This approach is further used to quantify 3-trifluoromethyl-4-nitrophenol (TFM) reductive metabolism. TFM is a widely used pesticide for the population control of sea lamprey (Petromyzon marinus), an invasive species of the Laurentian Great Lakes. Three animal models, sea lamprey, lake sturgeon (Acipenser fulvescens), and rainbow trout (Oncorhynchus mykiss), were selected to evaluate TFM reductive metabolism because they have been known to show differential susceptibilities to TFM toxicity. Amino-TFM (aTFM; 3-trifluoromethyl-4-aminophenol) was the only reductive metabolite identified through liquid chromatography-high-resolution mass spectrometry screening of liver extracts incubated with TFM and was targeted for electrochemical synthesis. After synthesis and purification, aTFM was used to develop a quantitative assay of the reductive metabolism of TFM through liquid chromatography and tandem mass spectrometry. The concentrations of aTFM were measured from TFM-treated cellular fractions, including cytosolic, nuclear, membrane, and mitochondrial protein extracts. Sea lamprey extracts produced the highest concentrations (500 ng/mL) of aTFM. In addition, sea lamprey and sturgeon cytosolic extracts showed concentrations of aTFM substantially higher than those of rainbow trout. However, other fractions of lake sturgeon extracts tend to show aTFM concentrations similar to those of rainbow trout but not with sea lamprey. These data suggest that the level of reductive metabolism of TFM may be associated with the sensitivities of the animals to this particular pesticide.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region><settlement><li>East Lansing</li></settlement><orgName><li>Université d'État du Michigan</li></orgName></list><tree><country name="États-Unis"><region name="Michigan"><name sortKey="Bussy, Ugo" sort="Bussy, Ugo" uniqKey="Bussy U" first="Ugo" last="Bussy">Ugo Bussy</name></region><name sortKey="Chung Davidson, Yu Wen" sort="Chung Davidson, Yu Wen" uniqKey="Chung Davidson Y" first="Yu-Wen" last="Chung-Davidson">Yu-Wen Chung-Davidson</name><name sortKey="Li, Ke" sort="Li, Ke" uniqKey="Li K" first="Ke" last="Li">Ke Li</name><name sortKey="Li, Weiming" sort="Li, Weiming" uniqKey="Li W" first="Weiming" last="Li">Weiming Li</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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